Increased High-Density Lipoprotein-3 Binding to Leukocytes Following Weight Loss and Improved Glycemic Control in Type 2 Diabetic Patients

Abstract

This study evaluates the effect on high-density lipoprotein (HDL) binding activity in cultured granulocytes before and after metabolic control of non-insulin-dependent diabetes mellitus ([NIDDM] type 2 diabetes) patients. In 20 type 2 diabetic patients, diabetic control was accomplished by administration of oral antidiabetic agents and dietary restrictions. Adequate metabolic control was reflected by a decrease in the fasting glucose, glycosylated hemoglobin (HbA1c), mean insulin, and body mass index (BMI). After control of the diabetes, the mean HDL3 cholesterol was increased from 0.918 ± 0.05 to 1.008 ± 0.05 mmol/L (P < .05) and apolipoprotein AI (apo AI) was increased from 103 ± 5.8 to 115 ± 5.1 mg/dL (P < .01). The HDL3 maximum specific binding was higher after versus before diabetic control, 77 ± 6 versus 122 ± 8 ng/mg cell protein (P < .01). This increase was related to an increase in maximum binding (Bmax from 4.97 × 10^-10 to 8.3 × 10^-10 mol/L, P < .001), and no significant changes were observed in the Kd (from 1.47 × 10^-7 to 2.04 × 10^-7 mol/L). These results suggest that the metabolic control of type 2 diabetes increases HDL3 binding activity.

Es la versión preprint del artículo. Se puede consultar la versión final en https://doi.org/10.1053/meta.2000.6248